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Silver in PDB 7so8: Crystal Structure of Glutathione S-Transferase From Shrimp Litopenaeus Vannamei in Complex with Silver Ions and A Molecules of Glutathione Binding in G-Site and H-Site

Enzymatic activity of Crystal Structure of Glutathione S-Transferase From Shrimp Litopenaeus Vannamei in Complex with Silver Ions and A Molecules of Glutathione Binding in G-Site and H-Site

All present enzymatic activity of Crystal Structure of Glutathione S-Transferase From Shrimp Litopenaeus Vannamei in Complex with Silver Ions and A Molecules of Glutathione Binding in G-Site and H-Site:
2.5.1.18;

Protein crystallography data

The structure of Crystal Structure of Glutathione S-Transferase From Shrimp Litopenaeus Vannamei in Complex with Silver Ions and A Molecules of Glutathione Binding in G-Site and H-Site, PDB code: 7so8 was solved by A.Escudero-Garcia, E.Rudino-Pinera, R.Miranda-Blancas, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 56.37 / 2.20
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 57.39, 93.02, 169.11, 90, 90.75, 90
R / Rfree (%) 19.1 / 26

Silver Binding Sites:

The binding sites of Silver atom in the Crystal Structure of Glutathione S-Transferase From Shrimp Litopenaeus Vannamei in Complex with Silver Ions and A Molecules of Glutathione Binding in G-Site and H-Site (pdb code 7so8). This binding sites where shown within 5.0 Angstroms radius around Silver atom.
In total 3 binding sites of Silver where determined in the Crystal Structure of Glutathione S-Transferase From Shrimp Litopenaeus Vannamei in Complex with Silver Ions and A Molecules of Glutathione Binding in G-Site and H-Site, PDB code: 7so8:
Jump to Silver binding site number: 1; 2; 3;

Silver binding site 1 out of 3 in 7so8

Go back to Silver Binding Sites List in 7so8
Silver binding site 1 out of 3 in the Crystal Structure of Glutathione S-Transferase From Shrimp Litopenaeus Vannamei in Complex with Silver Ions and A Molecules of Glutathione Binding in G-Site and H-Site


Mono view


Stereo pair view

A full contact list of Silver with other atoms in the Ag binding site number 1 of Crystal Structure of Glutathione S-Transferase From Shrimp Litopenaeus Vannamei in Complex with Silver Ions and A Molecules of Glutathione Binding in G-Site and H-Site within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Ag301

b:134.3
occ:1.00
OH E:TYR116 3.0 10.2 1.0
O E:HOH472 3.3 14.9 1.0
OG1 E:THR10 3.3 18.0 1.0
CD1 E:TRP8 3.6 10.2 1.0
ND2 E:ASN208 3.6 17.1 1.0
OH E:TYR7 3.6 10.8 1.0
SG2 E:GSH302 3.6 39.3 1.0
CB E:ASN208 3.8 15.5 1.0
CB E:ALA12 4.2 9.5 1.0
CB E:THR10 4.2 16.3 1.0
CG E:ASN208 4.2 15.9 1.0
N E:ASN208 4.2 12.5 1.0
CZ E:TYR7 4.2 15.1 1.0
CZ E:TYR116 4.2 16.2 1.0
CG E:TRP8 4.3 11.6 1.0
CG2 E:THR10 4.3 10.2 1.0
CE2 E:TYR7 4.3 14.7 1.0
NE1 E:TRP8 4.4 8.9 1.0
CB E:TRP8 4.5 14.1 1.0
CA E:ASN208 4.5 12.2 1.0
CE2 E:TYR116 4.8 18.9 1.0
O E:HOH547 4.9 28.9 1.0
OH E:TYR210 5.0 18.3 1.0

Silver binding site 2 out of 3 in 7so8

Go back to Silver Binding Sites List in 7so8
Silver binding site 2 out of 3 in the Crystal Structure of Glutathione S-Transferase From Shrimp Litopenaeus Vannamei in Complex with Silver Ions and A Molecules of Glutathione Binding in G-Site and H-Site


Mono view


Stereo pair view

A full contact list of Silver with other atoms in the Ag binding site number 2 of Crystal Structure of Glutathione S-Transferase From Shrimp Litopenaeus Vannamei in Complex with Silver Ions and A Molecules of Glutathione Binding in G-Site and H-Site within 5.0Å range:
probe atom residue distance (Å) B Occ
G:Ag301

b:88.6
occ:1.00
O G:HOH412 3.2 13.0 1.0
OH G:TYR116 3.3 18.3 1.0
OH G:TYR210 3.6 16.2 1.0
O12 G:GSH302 3.9 42.7 1.0
ND2 G:ASN208 3.9 4.8 1.0
CZ G:TYR116 3.9 17.9 1.0
CE2 G:TYR116 4.0 16.6 1.0
O11 G:GSH302 4.0 58.2 1.0
CB G:ASN208 4.1 11.2 1.0
C1 G:GSH302 4.1 53.6 1.0
SG2 G:GSH302 4.2 44.3 1.0
CA2 G:GSH302 4.2 57.0 1.0
OE1 G:GSH302 4.2 64.2 1.0
NE1 G:TRP8 4.2 10.7 1.0
CD1 G:TRP8 4.2 14.8 1.0
O2 G:GSH302 4.3 37.1 1.0
CZ G:TYR210 4.5 18.9 1.0
CD1 G:GSH302 4.6 74.5 1.0
CG G:ASN208 4.6 12.7 1.0
CE1 G:TYR210 4.7 12.8 1.0
OH G:TYR7 4.7 14.6 1.0
C2 G:GSH302 4.7 50.7 1.0
CB2 G:GSH302 4.8 43.5 1.0
N2 G:GSH302 4.9 86.2 1.0

Silver binding site 3 out of 3 in 7so8

Go back to Silver Binding Sites List in 7so8
Silver binding site 3 out of 3 in the Crystal Structure of Glutathione S-Transferase From Shrimp Litopenaeus Vannamei in Complex with Silver Ions and A Molecules of Glutathione Binding in G-Site and H-Site


Mono view


Stereo pair view

A full contact list of Silver with other atoms in the Ag binding site number 3 of Crystal Structure of Glutathione S-Transferase From Shrimp Litopenaeus Vannamei in Complex with Silver Ions and A Molecules of Glutathione Binding in G-Site and H-Site within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Ag301

b:132.2
occ:1.00
N2 D:GSH302 2.9 73.6 1.0
O D:HOH445 3.3 14.2 1.0
OH D:TYR210 3.5 23.0 1.0
SG2 D:GSH302 3.5 84.6 1.0
OH D:TYR116 3.6 16.8 1.0
CD1 D:GSH302 3.7 38.3 1.0
CA2 D:GSH302 3.7 80.5 1.0
OE1 D:GSH302 3.8 35.4 1.0
C2 D:GSH302 3.8 73.4 1.0
CE2 D:TYR116 3.8 26.9 1.0
CZ D:TYR116 4.0 26.3 1.0
N3 D:GSH302 4.0 49.2 1.0
CB2 D:GSH302 4.2 53.4 1.0
O2 D:GSH302 4.3 60.7 1.0
ND2 D:ASN208 4.3 23.7 1.0
NE1 D:TRP8 4.3 13.6 1.0
CB D:ASN208 4.4 18.2 1.0
CD1 D:TRP8 4.4 16.3 1.0
CZ D:TYR210 4.4 18.9 1.0
N1 D:GSH302 4.4 26.8 1.0
CE1 D:TYR210 4.6 19.1 1.0
CA3 D:GSH302 4.6 42.6 1.0
CD2 D:TYR116 4.8 18.5 1.0
CG D:ASN208 4.9 28.5 1.0

Reference:

A.Escudero-Garcia, E.Rudino-Pinera. Inhibition of Gst Class Mu of the Shrimp Litopenaeus Vannamei By Binding Silver Ions in H-Site. To Be Published.
Page generated: Wed Jul 10 09:02:05 2024

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